Abstract
Promoting cell growth is demanded in various applications, such as drug screening and regenerative medicine. Recently, non-thermal atmospheric pressure plasma (NTAPP), which can generate reactive oxygen and nitrogen species under atmospheric conditions, has been used to promote cell growth. In this study, microperfusion systems using the stimulation of NTAPP exposure were developed to induce cell growth. NTAPP was generated through the dielectric barrier discharge method. When NTAPP exposure was applied to pure water for 4 min, concentrations of nitrate, nitrite, and hydrogen peroxide reached 250, 20, and 0.5 mg l−1, respectively. Further, C2C12 murine myoblast cells were injected into the microperfusion system, stimulated with NTAPP exposure, and cultured for 3 d. Then, the liquid medium was changed to initiate cell differentiation. The formation of tubular structures was observed after incubation for 3 d.
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