Abstract
BackgroundMicrocystins are waterborne environmental toxins that induce oxidative stress and cause injuries in the heart. On the other hand, many physiological processes, including antioxidant defense, are under precise control by the mammalian circadian clock.ResultsIn the present study, we evaluated the effect of microcystin-LR (MC-LR) on the rhythmic expression patterns of circadian and antioxidant genes in rat cardiomyocytes using the serum shock technique. We found that a non-toxic dose (10 μm) of MC-LR decreased the amplitudes of rhythmic patterns of clock genes, while it increased the expression levels of antioxidant genes.ConclusionsOur results indicate an influence of MC-LR on the circadian clock system and clock-controlled antioxidant genes, which will shed some light on the explanation of heart toxicity induced by MC-LR from the viewpoint of chronobiology.
Highlights
A great number of physiological events in living organisms exhibit 24-h circadian rhythm fluctuations [1,2,3]
The central clock is located in the hypothalamic suprachiasmatic nucleus (SCN), while slave oscillators are found in various tissues, which can be driven by the central clock via behavioral and neuroendocrine signals [4]
We first investigated the toxicity of MC-LR towards rat H9C2 cardiomyocytes by measuring cell viability using the MTT test
Summary
A great number of physiological events in living organisms exhibit 24-h circadian rhythm fluctuations [1,2,3]. These intrinsic biological rhythms are driven by a circadian clock and are dominantly governed by light-dark (LD) and feeding cycles. The mammalian circadian clocks comprise a central clock and slave oscillators. The central clock is located in the hypothalamic suprachiasmatic nucleus (SCN), while slave oscillators are found in various tissues, which can be driven by the central clock via behavioral and neuroendocrine signals [4]. Autoregulatory transcriptional translational feedback loops involving several clock genes build up intracellular clocks. Many physiological processes, including antioxidant defense, are under precise control by the mammalian circadian clock
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