Abstract
ABSTRACTZinc sulfide (ZnS) nanoparticles were synthesized in a single-step process using Desulfovibrio desulfuricans at room temperature and atmospheric pressure. The kinetics of ZnS biosynthesis and the cellular distribution of Zn were also studied by measuring the Zn2+ and ZnS concentrations. The microstructure of the ZnS nanoparticles was characterized by means of high-resolution transmission electron microscopy and X-ray diffraction. The particles were essentially granular, and the particle size ranged from approximately 5 to 8 nm. The total UV–Vis spectrum of the product migrated toward blue wavelengths compared with the ultraviolet absorption wavelength of the ZnS bulk material. The yield of ZnS nanoparticles was 97.8% after 96 h. A mechanistic experiment showed that ZnS nanoparticles were produced inside the cells and released into the solution. In the cells, Zn was the most highly concentrated in the membrane, followed by the cell wall and cytoplasm.
Published Version
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