Microbial regulation of nitrous oxide emissions from chloropicrin-fumigated soil amended with biochar

Abstract

The microbial mechanism underpinning biochar’s ability to reduce emissions of the potent greenhouse gas nitrous oxide (N2O) is little understood. We combined high-throughput gene sequencing with a dual-label 15N–18O isotope to examine microbial mechanisms operative in biochar made from Crofton Weed (BC1) or pine wood pellets (BC2) and the N2O emissions from those biochar materials when present in chloropicrin (CP)-fumigated soil. Both BC1 and BC2 reduced N2O total emissions by 62.9–71.9% and 48.8–52.0% in CP-fumigated soil, respectively. During the 7-day fumigation phase, however, both BC1 and BC2 increased N2O production by significantly promoting nirKS and norBC gene abundance, which indicated that the N2O emission pathway had switched from heterotrophic denitrification to nitrifier denitrification. During the post-fumigation phase, BC1 and BC2 significantly decreased N2O production as insufficient nitrogen was available to support rapid population increases of nitrifying or denitrifying bacteria. BC1 and BC2 significantly reduced CP’s inhibition of nitrifying archaeal bacteria (AOA, AOB) and the denitrifying bacterial genes (nirS, nirK, nosZ), which promoted those bacterial populations in fumigated soil to similar levels observed in unfumigated soil. Our study provided insight on the impact of biochar and microbes on N2O emissions.