Microbial mediated synthesis of ZnO nanoparticles derived from Lactobacillus spp: Characterizations, antimicrobial and biocompatibility efficiencies

Abstract

The present study reported that the green protocols for the synthesis of ZnO nanoparticles with Lactobacillus spp extract are cost-effective and eco-friendly and the reports say that green ZnO nanoparticles have better antimicrobial and biocompatibility analysis. X-ray diffraction (XRD), Ultraviolet absorption spectrum (UV–Vis), Fourier transform infrared spectroscopy (FTIR), Field emission scanning electron microscopy (FE-SEM), Energy dispersive X-ray analysis (EDX), and Atomic force microscopy (AFM) were used to characterize the ZnO nanoparticles. XRD and FTIR analysis confirmed the formation of a pure ZnO phase. The antimicrobial efficiencies have been conducted using ZnO nanoparticles against microbial strains such as Clostridium difficile, Clostridium perfringens, E. coli, Salmonella typhi, Candida albicans, and Aspergillus flavus using the agar well method. The biocompatibility of the human colon cancer cell line (HT-29) was performed by MTT assay. Results of this study, MTT test measurements on cell viability studies have demonstrated the excellent biocompatibility activity against HT 29 cancer cell line. The effective antimicrobial activity of ZnO nanoparticles against different Gram positive and Gram negative bacterial and fungal pathogens was demonstrated by the amazing inhibition zones (mm). In this research, we concluded that bio-mediated ZnO nanostructures proved to be an excellent new antimicrobial and anticancer material.