Abstract
The hydrolysis of the herbicide diclofop-methyl, methyl ( ± 2-[4-(2,4-dichlorophenoxy)-phenoxy] propanoic acid, methyl ester) was determined in an enzyme solution and in two soils with or without the addition of three plasmolytic agents. In the absence of soil, propylene oxide had no effect on hydrolytic activity of esterase whereas sodium azide and toluene decreased the activity of the enzyme to hydrolyze the herbicide. In soil, the rate of hydrolysis of diclofop-methyl followed the order pH 6.4 > 7.5 ⩾ 5.5. Propylene oxide fumigation and autoclaving the soil inhibited hydrolysis. The ester was hydrolyzed faster in soil (41% in 2 h) than in its phosphate buffer extract (46% in 16 h). Diclofop-methyl appears to be hydrolyzed in soil by hydrolase enzymes associated with viable microorganisms.
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