Abstract
Escherichia coli JM109 (pGV3-SBA) can assimilate starch by fusing the starch-digesting enzyme α-amylase from Streptococcus bovis NRIC1535 to an OprI' lipoprotein anchor on the cell membrane. This study shows microbial fuel cells (MFCs) development using this recombinant type of E.coli and starch as fuel. We observed the current generation of MFCs with E.coli JM109 (pGV3-SBA) for 120h. During this period, it consumed 7.1g/L of starch. A mediator in the form of anthraquinone-2,6-disulfonic acid disodium salt at 0.2, 0.4, and 0.8mM was added to the MFCs. The highest maximum-current density (271mA/m2) and maximum-power density (29.3mW/m2) performances occurred in the 0.4mM mediator solution. Coulomb yields were calculated as 3.4%, 3.0%, and 3.5% in 1.0, 5.0, and 10.0g/L of initial starch, respectively. The concentrations of acetic acid, succinic acid, fumaric acid, and ethanol as metabolites were determined. In particular, 38.3mM of ethanol was produced from 7.1g/L of starch. This study suggests the use of recombinant E.coli which can assimilate starch present in starch-fueled MFCs. Moreover, it proposes the possibility of gene recombination technology for using wide variety of biomass as fuel and improving MFC's performance.
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