Microbial Communities in Anaerobic Acidification-Denitrification and Methanogenesis Process for Cassava Stillage Treatment

Abstract

This study investigated operational performance and microbial communities in the integrated acidification-denitrification bioreactor and the followed methanogenesis process. Industrial wastewater, cassava stillage (CS) was used as the carbon source amended with or without nitrate. The results showed that acidification and denitrification could occur simultaneously in a single acidification-denitrification reactor, and denitrificatoin did not suppress the acidogenic activity. Both denitrification and DNRA could contribute to nitrate reduction and proportions of them were about 60% and 40% respectively at the tested condition of COD/NO3-Nof 50. The introduction of nitrate into acidogenic phase did not have any effect on the followed methanogenic process. Microbial communities sampled from two systems were analyzed by culture-independent techniques based on PCR-DGGE. The relative abundance of acid-producing bacteria (primarily Parabacteroides distasonis and Chloroflexi) in the nitrate-amended reactor further confirmed that the addition of nitrate did not suppress the activity of acid-producing bacteria. Bacteria involved in denitrification and DNRA were also detected. The archaeal communities in methanogenic reactors of two systems showed no significant differences. And Methanoculleus and Methanolobus were the dominant bacteria in the culture.