Abstract
Here, we present a metagenomic analysis of the microflora of the surface waters of the Shardara reservoir, the largest artificial reservoir in Southern Kazakhstan, created to meet irrigation and hydropower engineering needs. In this case, shotgun metagenomic sequencing of the microbial community DNA was used.
Highlights
We present a metagenomic analysis of the microflora of the surface waters of the Shardara reservoir, the largest artificial reservoir in Southern Kazakhstan, created to meet irrigation and hydropower engineering needs
Each of the water samples was immediately filtered using a cellulose membrane with a diameter of 300 mm and pore size of 3 m; the filtrate was concentrated to a volume of 500 ml using tangential flow filtration (Vivaflow 200, Sartorius, with a 200-cm2 polyethersulfone membrane)
DNA libraries were prepared from 1 ng of the isolated nucleic acids using the Nextera XT DNA sample preparation kit (Illumina, USA) in accordance with the instructions
Summary
We present a metagenomic analysis of the microflora of the surface waters of the Shardara reservoir, the largest artificial reservoir in Southern Kazakhstan, created to meet irrigation and hydropower engineering needs. Each of the water samples (sample volume, 5 liters) was immediately filtered using a cellulose membrane with a diameter of 300 mm and pore size of 3 m; the filtrate was concentrated to a volume of 500 ml using tangential flow filtration (Vivaflow 200, Sartorius, with a 200-cm2 polyethersulfone membrane).
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