Abstract
Gegen Qinlian decoction (GGQLD) has a definite effect on T2DM in clinic, and it has the effect of lowering blood sugar, improving insulin resistance, and improving the blood lipid level of rats with dyslipidemia, but the intervention mechanism of GGQLD on dyslipidemia has not been clarified. The changes in endogenous metabolites in the plasma of high-fat diet-induced dyslipidemia rats treated with Ge Gen Qin Lian Decoction (GGQLD) were studied to elucidate the therapeutic effects and mechanism of action of GGQLD in dyslipidemia. Based on ultrahigh-performance liquid chromatography coupled with quadrupole-time-of-flight tandem mass spectrometry (UHPLC-Q-TOF-MS), the metabolic profiles of rat serum samples were collected. The rat model of dyslipidemia was induced by a 60% fat-fed high-fat diet. After feeding the rats with a high-fat diet for 4 weeks, dyslipidemia appeared. After 5 weeks of GGQLD (14.85 g kg−1) administration, the metabonomics of rats' plasma samples in the normal group, model group, and administration group were analyzed. Mass profiler professional (MPP), SIMCA-P 14.1, and Graphpad prism 6.0 software were used combined with METLIN biological database and human metabolite database HMDB to screen and identify endogenous biomarkers. Metaboanalyst 4.0 software was used by combining with HMDB and KEGG databases; the enrichment and metabolic pathway of biomarkers were analyzed to explore the metabolic mechanism of dyslipidemia rats induced by high-fat diet and the intervention mechanism of Gegen Qinlian decoction. After 5 weeks of administration of GGQLD, the levels of serum TC and TG were significantly decreased (P < 0.05, P < 0.01), while HDL-C and LDL-C were not significantly affected. After administration, the food intake of rats in the administration group decreased gradually, and the change trend of body weight gradually slowed down. The metabonomics of rat plasma samples results showed that 23 potential biomarkers including α-linolenic acid, arachidonic acid, and lysophosphatidylcholine were significantly changed in positive ion mode. Studies have shown that GGQLD has a significant lipid-lowering effect on dyslipidemia rats induced by a high-fat diet, and its preventive mechanism is related to tryptophan metabolism, fatty acid biosynthesis, α-linolenic acid metabolism, arachidonic acid, and glycerophosphatidyl metabolism pathway.
Highlights
Type 2 diabetes mellitus (T2DM) accounts for 90% of the total population of diabetes mellitus, but most patients do not have obvious symptoms, especially in the early stage of diabetes mellitus, which is accompanied by varying degrees of overweight or obesity and dyslipidemia
Dyslipidemia refers to the disorder of lipid metabolism in the body and the Evidence-Based Complementary and Alternative Medicine disorder of plasma lipoprotein exceeding the normal range, accompanied by the increase of total cholesterol (TC), triglyceride (TG), and low-density lipoprotein-cholesterol (LDL-C) and the decrease of high-density lipoproteincholesterol (HDL-C)
Establishment of the Rat Model with Dyslipidemia and Changes of Blood Lipid Level in Rats after 5 Weeks of Gegen Qinlian Decoction Administration. after feeding a 60% fatfed high-fat diet for 4 weeks, the weight of rats increased significantly, there was a significant difference in Lee’s Indices of rats between the model group and the control group, TC and LDL-C increased significantly, HDL-C decreased significantly, and TG increased without statistical difference
Summary
Type 2 diabetes mellitus (T2DM) accounts for 90% of the total population of diabetes mellitus, but most patients do not have obvious symptoms, especially in the early stage of diabetes mellitus, which is accompanied by varying degrees of overweight or obesity and dyslipidemia. With the popularization of Western fast food, high-fat and high-calorie diet and other bad habits, dyslipidemia and obesity have become a common phenomenon and the incidence rate of dyslipidemia in T2DM patients is 50% [1]. Dyslipidemia is a risk factor for diabetic complications [3,4]. Adiels et al [6] studied showed that dyslipidemia is an early event of T2DM. E results of the UK Prospective Diabetes Study (UKPDS) show that dyslipidemia is an independent risk factor of T2DM, and there is significant dyslipidemia in the early stage of the disease Adiels et al [6] studied showed that dyslipidemia is an early event of T2DM. e results of the UK Prospective Diabetes Study (UKPDS) show that dyslipidemia is an independent risk factor of T2DM, and there is significant dyslipidemia in the early stage of the disease
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