Metabolomic and microbiomic resilience of Hong Kong oysters to dual stressors: Zinc oxide nanoparticles and low salinity

Abstract

Zinc oxide nanoparticles, increasingly used in industrial and consumer products, and low salinity, exacerbated by climate change-induced alterations in precipitation patterns, represent significant environmental pressures in estuarine and coastal environments. This study advances previous research on their impacts on Hong Kong oysters (Crassostrea hongkongensis) by integrating metabolomics of hepatopancreas and gills with intestinal microbiomics. Employing advanced multi-omics integration methods, our analysis reveals novel insights into metabolic resilience under combined stress conditions. This resilience is characterized by coordinated, organ-specific adjustments in energy metabolism (d-glucose 1-phosphate in hepatopancreas, cytidine in gills), antioxidant defenses (glutathione, meso-2,6-diaminoheptanedioate, pimelic acid in hepatopancreas; indole, 3-(3-hydroxyphenyl)propanoic acid in gills), immune function (l-glutamine, ergocalciferol in hepatopancreas; argininosuccinic acid in gills), and membrane stability (lanosterin in hepatopancreas, allantoin in gills). Notably, under dual stressors, we observed a previously undescribed stabilization of microbial alpha diversity and certain phyla, an absence of distinctive biomarkers, and certain metabolic activity stabilization within the intestinal microbiota. These findings suggest robust compensatory mechanisms that maintain physiological homeostasis and microbial balance under stress, contrasting with primarily negative impacts reported in previous studies. Integration of metabolomic and microbiomic data revealed coordinated responses between microbial community changes and metabolic adjustments, particularly in osmoregulation, energy metabolism and antioxidant defenses, under dual stressors. This comprehensive approach provides a more realistic model of environmental challenges, revealing sophisticated adaptive strategies in Hong Kong oysters. Our study offers critical insights for understanding bivalve resilience, informing conservation strategies, and managing marine ecosystems in the face of increasing anthropogenic pressures.