Abstract
The metabolism of glutamine by isolated, respiring rat liver mitochondria produced ammonia and asparate as the main identified products. The dependence of ammonia and aspartate production on glutamine concentration was sigmoidal. Glutaminase activity was located in the inner membrane plus matrix subfraction of liver mitochondria. Rotenone inhibited glutaminase activity in whole mitochondria; addition of succinate did not remove the inhibition. Glutamine and glutamate at low concentrations were metabolized, to some extent independently, to form ammonia and aspartate. It was concluded that the metabolism of both glutamine and glutamate is integrated with the tricarboxylic acid cycle to produce the two precursors of urea, ammonia and aspartate.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
