Abstract
Abstract The utilization of α-Ketoglutaric acid in human erythrocytes was enhanced by adding arsenite, cadmium ions, alanine, aspartic acid, and glutamine, but was not changed after addition of glycine, lcucine, valine, histidine, and tryptophan. Glutamic acid, ammonium ions, and isonicotinylhydrazide inhibited the utilization of α-Ketoglutaric acid. During incubation of erythrocytes with α-Ketoglutaric acid the level of glutamic acid rose while that of aspartic acid and tyrosine decreased. It was further found that an addition of α-ketoglutaric acid did not appreciably affect the formation of carbon dioxide and uptake of oxygen. In the presence of methylene blue and α-ketoglutarate, no oxygen was utilized in the absence of glucose. On the basis of these results it can be concluded that the main pathway of α-ketoglutarate degradation in erythrocytes is its conversion to glutamic acid mediated by transaminases.
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