Abstract
1. 1. Saccharomyces cerevisiae grown in the presence of 2-deoxy- d-glucose incorporate this glucose and mannose analogue into cell wall polysaccharides. Fractionation of cell walls to mannan- and glucan-containing fractions followed by analysis for glucose, mannose and deoxyglucose showed that deoxyglucose was incorported mainly, if not exclusively, into cell wall mannan. 2. 2. Mild acid hydrolysis of the mannan-containing fraction isolated from deoxyglucose-grown cells afforded besides free deoxyglucose two oligosaccharides containing both mannose and deoxyglucose. They were tentatively identified as 3-O-α, d-mannopyranosyl-2-deoxy- d-glucose and a trisaccharide containing the former disaccharide and an additional mannose residue at non reducing terminal. 3. 3. The results demonstrate direct interaction of deoxyglucose metabolites with the enzyme system of Saccharomyces cerevisiae responsible for the cell-wall mannan biosynthesis.
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