Abstract
Selenium (Se) was found to inhibit the growth of the yeast Candida utilis ATCC 9950. Cells cultured in 30 mg selenite/L supplemented medium could bind 1368 µg Se/g of dry weight in their structures. Increased accumulation of trehalose and glycogen was observed, which indicated cell response to stress conditions. The activity of antioxidative enzymes (glutathione peroxidase, glutathione reductase, thioredoxin reductase, and glutathione S-transferase) was significantly higher than that of the control without Se addition. Most Se was bound to water-insoluble protein fraction; in addition, the yeast produced 20–30 nm Se nanoparticles (SeNPs). Part of Se was metabolized to selenomethionine (10%) and selenocysteine (20%). The HPLC-ESI-Orbitrap MS analysis showed the presence of five Se compounds combined with glutathione in the yeast. The obtained results form the basis for further research on the mechanisms of Se metabolism in yeast cells.
Highlights
Selenium (Se) is an important element involved in many metabolic processes [1]
Candida utilis ATCC 9950 C. utilis ATCC 9950 was able to grow in control and Se-supplemented media
This could be due to the inhibitory effect of Se on the reproduction of yeast cells
Summary
Selenium (Se) is an important element involved in many metabolic processes [1]. It has been recognized as an essential nutrient for its antioxidant properties [2], and it plays an important role in thyroid hormone metabolism and immune defense mechanisms [3]. The gap between the essentiality and the toxicity of Se is narrow and depends on the chemical form [7]. The metabolism of Se in some plants is induced in the context of bioremediation and as a source of phytochemicals for new potential therapeutic agents. The principal chemical forms of Se in plants include selenomethionine (SeMet), methyselenocysteine, and glutamylselenomethyl-selenocysteine [8]
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