Abstract
Batch cultures of Clostridium acetobutylicum, were examined with 0, 0.1 and 1 mM methyl viologen addition at four different controlled pH values (between 4.5 and 6.5). Methyl viologen addition diverted the electron flow: reducing equivalents normally released as molecular hydrogen were directed towards NAD(P)H formation. Production of butanol, the most reduced non-gaseous product, was sharply increased (0.65 mol/mol glucose) at the expense of acetone and butyric and acetic acids. In addition to butanol and lactate production, NADH excess induced the formation of glycerol, a product that has never been reported to be formed by C. acetobutylicum. Metabolic perturbation brought about by the electron carrier led to a reduction of the growth rate and an increase of the lag phase. A correlation between the shape of the redox potential curve and the switch from an acidogenic to a solventogenic metabolism is reported.
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