Abstract
BackgroundWhile pars plana vitrectomy (PPV) has become the third most commonly performed surgery in the world, it can also induce multiple post complications easily. Among them, cataract progression is the most frequent one that can lead to blindness eventually.MethodsTo understand the underlying mechanisms of post PPV cataract progression, we performed comprehensive metabolic characterization of aqueous humor (AH) samples from 20 cataract patients (10 post PPV complication and 10 none PPV cataract) by a non-targeted metabolomic analysis using gas chromatography combined with time-of-flight mass spectrometer (GC/TOF MS).ResultsA total of 263 metabolites were identified and eight of them are determined to be significantly different (VIP ≥ 1 and p ≤ 0.05) between post PPV group and none PPV control group. The significantly changed metabolites included glutaric acid and pelargonic acid that play key roles in the regulation of oxidative stress and inflammatory responses. Furthermore, we constructed a metabolic regulatory network in each group based on metabolite-metabolite correlations, which reveals key metabolic pathways and regulatory elements including amino acids and lipids metabolisms that are related to cataract progression.ConclusionsAltogether, this work discovered some potential metabolite biomarkers for post PPV cataract diagnostics, as well as casted some novel insights into the underlying mechanisms of cataract progression after PPV.
Highlights
While pars plana vitrectomy (PPV) has become the third most commonly performed surgery in the world, it can induce multiple post complications
In order to reveal the underlying mechanism in the cataract progression after Pars plana vitrectomy (PPV), in the present study by using the same technology, we collected 20 aqueous humor samples from 20 cataract patients
In conclusion, taking advantage of an unbiased technology GC-TOF-Gas chromatography coupled to time-of-flight mass spectrometer (MS), we fully showed the metabolic characterization of aqueous humor (AH) in patients after PPV
Summary
While pars plana vitrectomy (PPV) has become the third most commonly performed surgery in the world, it can induce multiple post complications . Nuclear sclerotic cataract progression after PPV has been known for many years, Metabolomics analysis can provide comparative, semiquantitative information of a large number of metabolites in biological samples from different groups at a specific time [6]. In recent years, this powerful –omics technology has been applied in ophthalmology researches such as diabetic retinopathy, glaucoma, and cataract progression, aiming to identify metabolic biomarkers and pathways that are important for understanding disease mechanisms and developing novel diagnosis and therapies [6,7,8].
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