Metabolic and metatranscriptional characteristics of corals bleaching induced by the most severe marine heatwaves in the South China Sea

Abstract

Coral bleaching significantly affects the function and health of coral reef ecosystems; however, the mechanisms underlying metabolism and transcription in corals remain unclear. In this study, untargeted metabolomics and metatranscriptomic analyses were performed to analyze the differences between unbleached and bleached Pocillopora corals during the most severe marine heatwaves. Difference analysis showed that bleached corals had significant metabolomic characteristics compared with those in unbleached corals. These differences were significant (p < 0.05) according to partial least squares discriminant analysis (PLS-DA). Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis revealed that the metabolites were significantly enriched in numerous pathways in bleached or unbleached corals, such as steroid hormone biosynthesis, biosynthesis of unsaturated fatty acids, and pyrimidine metabolism. Bleaching greatly affects coral reproduction as well as the tolerance of coral symbionts to heat stress. In metatranscriptomic analysis, we observed large gene expression differences between unbleached and bleached corals. Three Gene Ontology directed acyclic graphs (DAGs) were constructed to show the significantly differentially expressed genes (DEGs). Many biological and molecular processes were significantly enriched between bleached corals to unbleached corals, such as metabolic processes, lipid metabolic processes, oxidation-reduction processes, single-organism metabolic processes, and protein metabolic processes. Metabolome and metatranscriptome analyses showed that bleaching caused substantial physiological damage to corals. This study provides insight into the metabolic and transcriptional changes that occur in corals during bleaching.