Abstract
To investigate the influence of partial colectomy associated with hepatectomy on the biodistribution of the (99m)Tc-phytate, on metabolic parameters, as well as labeling and morphology of red blood cells. Wistar rats were distributed into three groups (each with six), nominated as colectomy, colectomy+hepatectomy and sham. In the 30(th) postoperative day all rats were injected with (99m)Tc-phytate 0.1mL i.v. (radioactivity 0.66 MBq). After 15 minutes, liver sample was harvested and weighed. Percentage radioactivity per gram of tissue (%ATI/g) was determined using an automatic gamma-counter. Serum AST, ALT, alkaline phosphatase and red blood cells labeling were determined. The liver %ATI/g and red blood cells labeling were lower in colectomy and colectomy+hepatectomy rats than in sham rats (p <0.05), and no difference was detected comparing the colectomy and colectomy+hepatectomy groups. Red blood cells morphology did not differ among groups. Serum levels of AST, ALT and alkaline fosfatase were significantly higher in colectomy+hepatectomy than in colectomy rats (p<0.001). Hepatectomy associated with colectomy lowered the uptake of radiopharmaceutical in liver and in red blood cells in rats, coinciding with changes in liver enzymatic activity.
Highlights
The liver is affected by metastases in 50% of patients with colorectal cancer and metastatic disease remains the leading cause of cancer-related death
We have demonstrated relevant postoperative metabolic disorders after massive intestinal resection[4], total gastrectomy[5,6], total colectomy[7] and Roux-en-Y gastric bypass[8], by using this experimental model
Based on the above concepts, this study aimed to examine in an animal model if colectomy associated with hepatectomy modifies liver biodistribution of 99mTc-phytate, metabolic parameters and labeling of red blood cells
Summary
The liver is affected by metastases in 50% of patients with colorectal cancer and metastatic disease remains the leading cause of cancer-related death. Pertechnetate (99mTcO4-) is used in the diagnosis of gastric, kidney, liver, lung, intestinal, skeletal, endocrine, heart, blood flow and postoperative disorders[3]. Used intravenously, this radiopharmaceutical remains in the circulation long enough to analyse various organic and functional parameters for the diagnosis of diseases. This radiopharmaceutical remains in the circulation long enough to analyse various organic and functional parameters for the diagnosis of diseases It comes into equilibrium with the extracellular space, is taken up by tissues, and a small percentage is eliminated by urinary excretion. Some recent studies have demonstrated significant changes in biodistribution of 99mTcO4- on the postoperative of major surgery[4,5,6,7,8]
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