Metabolic alteration in bovine periodontal ligament and dental pulp mitochondria induced by bacterial endotoxin.

Abstract

In pursuit of the cellular pathogenicity of periodontal disease, subcellular effects of bacterial endotoxin (lipopolysaccharide: LPS) on the tricarboxylic acid (TCA) cycle were studied using mitochondrial fractions from bovine periodontal ligament and dental pulp. Mitochondrial fractions were incubated with succinate-1, 4-14C as radioactive substrate for analyses of organic and amino acids synthesized both with and without LPS.Addition of LPS caused obvious increase in amount synthesized, compared to control. Production of fumarate, α-ketoglutarate and malate increased 184, 330 and 362 percent, respectively, with addition of 100μg/ml E. coli 0127: B8 LPS.Glutamate, glycine, alanine and aspartate were the major amino acids synthesized from bovine periodontal ligament mitochondrial fraction. Production of glutamate increased 148, 174and133 percent with addition of 50, 100 and 200μg/ml LPS, respectively.These findings demonstrate that bacterial endotoxin can affect the process of periodontal disease by altering the metabolism and biosynthesis in mitochondria of the fibroblast.