Abstract
We collected data regarding 340 disease resistance quantitative trait loci (QTLs) from the maize genomic database (MaizeGDB). We constructed an integrated linkage map and analyzed this map by using the BioMercator 2.1 software with IBM2 2008 Neighbors genetic linkage map as a reference. We used a meta-analysis method to identify five "consensus" synthetic resistance QTLs located on maize chromosomes 1, 3, 6, and 10, with map intervals of 5.14, 9.00, 28.50, 1.73, and 33.34 cM, respectively. The gene and marker sequences within the five "consensus" QTLs were downloaded from the MaizeGDB website. We identified eight resistance gene analogs (RGAs), through comparison of these sequences with the resistance genes of other members of Poaceae by using the online BLASTx software. On the basis of comparative mapping between the maize genetic map and the rice physical map, 54 rice and 44 maize resistance genes were projected onto the maize IBM2 2008 Neighbors genetic linkage map by using a synteny conservation approach. Additionally, 15 resistance genes in the "consensus" QTL regions were found in two "consensus" QTLs on chromosomes 3 and 6; these resistance genes included rp3, scmv2, wsm2, RG3, RG16, RG36, RG51, RG53, scmv1, mdm1, RG5, RG8, RG10, RG14, and RG29. Our results provide valuable information for fine-mapping QTL, gene cloning, and molecular breeding for resistance in maize.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
