Abstract
Soon after their transcription, eukaryotic messenger RNA precursors (heterogeneous nuclear RNA) are assembled into ribonucleoprotein particles (hnRNP). In the present experiments we have asked whether the mRNA-homologous components of hnRNA are complexed with proteins in hnRNP or, alternatively, exist as stretches of naked RNA in the particles. Mild digestion of HeLa cell hnRNP particles by micrococcal nuclease, pancreatic RNAase or an endogenous particle-bound nuclease, followed by hybridization of the surviving RNA with a complementary DNA probe for poly(A) + mRNA sequences reveals that messengerhomologous sequences are protected from nuclease attack in hnRNP.
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