Abstract
It is important for a controlled release system to determine whether nanoparticles canpenetrate cell membranes and deliver protein into the nuclear or cytosolic compartments ofcells, and thus function as carriers. Here, we prepared different functionalizedmesoporous magnetic hollow nanoparticles (MMHs) and chose bovine serum albumin(BSA) as a model protein to detect the intracellular trafficking of MMHs. Theresults showed that MMHs modified with amino groups (AMMHs) were efficient inprotein loading and that the loading was dependent on the pH, temperature andionic strength. Furthermore, we found that the AMMHs not only transportedBSA into the cells but also released the BSA carried into the nuclear or cytosoliccompartments of the cells. In addition, the nanoparticles were biocompatible,and the encapsulation of BSA in AMMHs did not affect their bioactivity. Takentogether, AMMHs are excellent carriers for releasing protein into the cytosol andnucleus, and they have the potential to be used in a controlled release system.
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