Mercury can be transported into marine copepod by polystyrene nanoplastics but is not bioaccumulated: An increased risk?

Abstract

Plastic pollution is a serious problem in the global marine environment because it can produce negative effects at the biological and ecological levels. Due to large surface-area-to-volume ratio and inherent hydrophobicity, nanoplastics can serve as carriers of contaminants, and may affect their fate and toxicity in marine environments. However, the combined effects of nanoplastics and mercury (Hg) in marine organisms have not been well characterized. In this study, after verifying the ingestion of polystyrene nano-size plastics (PS NPs, 50 nm) by the copepod Tigriopus japonicus and adsorption of Hg to PS NPs, we investigated the effects of PS NPs and Hg exposure (alone or in combination) for 48 h on the copepods. Specifically, a 72-h depuration was performed after 48 h exposure. The results showed that after 48 h exposure, the copepod's Hg concentration was significantly increased in the combined exposure group compared to that in the Hg treatment group, but these differences did not persist following 24 h of depuration. Therefore, PS NPs transported Hg into the copepods but did not promote Hg bioaccumulation. Treatment with PS NPs alone did not induce toxicity in T. japonicus, but co-exposure to PS NPs and Hg resulted in elevated transcription of genes related to energy production, antioxidant response, and detoxification/stress defense when compared with Hg treatment alone, demonstrating the synergistic interaction between PS NPs and Hg. Our findings contribute to a comprehensive understanding about the combined toxicity of nanoplastics and metals and the potential ecological risks of associated with these effects in marine environments.