Abstract
Abstract Cassava (Manihot esculenta Crantz) is worldwide the sixth-largest food crop and highly drought resistant. The expression of the myeloblastosis (MYB) family protein MeMYB26 in cassava was previously reported to be significantly up-regulated under water deficit. To study the basic characterization, natural variations and potential functions of MeMYB26 for drought resistance in cassava, the protein was subjected to bioinformatics analysis, multiplex-PCR with next-generation sequencing and candidate association studies. The results indicated that MeMYB26 is a typical transcription factor, with two MYB DNA-binding and transcriptional activation domains. One stop-gained and five nonsynonymous variations in the genomic region of MeMYB26 were significantly associated with drought resistance traits. The results of the scale-free coexpression network showed that the MeMYB26 gene plays a critical role in plant stress resistance, growth and biomass development. It was concluded that MeMYB26 is a reliable candidate gene associated with drought tolerance and biomass storage in cassava.
Highlights
Cassava (Manihot esculenta Crantz) is an important food and energy crop (Vieira et al 2020) that is mainly planted in tropical and subtropical areas
MeMYB26 is a typical MYB family transcription factor To study the basic characterizations of MeMYB26, gene structure was analyzed by Splign
Three transformants of MeMYB26::pGBKT7/Y187 formed obvious blue plaque, while the plaque on the line of pGBKT7/Y187, the negative control, was milky white (Figure 1C). This indicates that MeMYB26 has a transcription activation domain (AD)
Summary
Cassava (Manihot esculenta Crantz) is an important food and energy crop (Vieira et al 2020) that is mainly planted in tropical and subtropical areas. Drought resistance has been a key focus of cassava breeding studies. The myeloblastosis (MYB) protein family is one of the largest transcription factor families in plants. Many MYB proteins have been reported to be involved in the resistance mechanism to dehydration stress in plants (Tan et al 2019). A total of 26 R2R3-MYB genes were responsive to both ethylene and drought stress signals (Liao et al 2016). Other studies reported 26 cassava MYB or MYB-related proteins that responded to drought and cold stress signals, 14 of which were up-regulated and 12 down-
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