Abstract
After fixation in an acid fixative, 20 μm paraffin sections of microsporocytes of Lilium longiflorum showed a number of details when observed by scanning electron microscopy. These were: (1) the surface of meiotic prophase chromosomes, (2) chromatids of synapsed pachytene chromosomes, (3) chromosome-nuclear boundary associations, (4) chromosome-nucleoli associations, (5) the surface of intact nucleoli, and (6) the interior of sectioned nucleoli. The most suitable fixative appears to be unbuffered 10% formalin, but 10:5:85 formalin-acetic-alcohol or 1:3 acetic-alcohol could be used successfully, since they solubilized the nucleoplasm. Critical point drying technique, compared with simple air drying, yielded no difference in the image of the chromosomes. The procedure described is useful for observing meiotic prophase from preleptotene through pachytene stages.
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