Mechanisms of cellular proliferation stimulation under the effect of L-amino acids in the tissue culture of young and old rats

Abstract

The statistical method of rank correlation was used to analyze the correlation of individual physicochemical characteristics of L-amino acids, as well as of dipole moments of the first pair of nucleotides of their codons, to the biological activity of amino acids, i.e. the stimulation of cellular proliferation. The experimental material was obtained from the organotypic tissues of ecto- and mesodermal origin in young and old rats. It was demonstrated that each of the stimulating amino acids acts 1.5–6.0 times less often in explants from old rats as compared to the explants from young rats. A weak correlation was detected between the stimulation of cellular proliferation and physicochemical characteristics (hydrophobicity, dipole moment and isoelectric point of the amino acids) in many tissues, but high and moderate correlation was detected in the brain subcortical explants, cerebellum and skin. However, a moderately close correlation was detected between the dipole moment of the first pair of nucleotides of their codons and the stimulating activity for explants of the brain subcortical structures (young and old rats), cerebellum and skin (young rats), and Spearman’s correlation coefficient (r s = 0.52; 0.45; 0.60; and 0.71, respectively). A moderate correlation was also detected for explants of the testis and cartilage. It can be suggested that some physicochemical and biochemical characteristics of amino acids correlate to their biological activity, which is especially remarkable in tissues derived from ectoderm.