Abstract
Objective To explore the mechanism underlying the inhibitory effects of Hydrotalcite on Helicobacter pylori (H.pylori). Methods H. pylori international standard strain 26695 was resuscitated and incubated in Columbia solid culture medium and brucella broth. It was divided into blank control group, H. pylori injured group and H. pylori with Hydrotalcite group. H. pylori were treated by Hydrotalcite, and the viability changes of H. pylori were observed. The morphological changes of H. pylori were detected by Gram′s stain. The changes of mycoproteins expression of H. pylori were examined by Coomassie blue stain. The effects of H. pylori on cell apoptosis of gastric epithelial cell line GES-1 was compared between groups with and without Hydrotalcite. Subcellular structure and attachment ability of H. pylori on GES-1 cells were observed with transmission electron microscopy and scanning electron microscopy. T test was performed for statistical analysis. Results Hydrotalcite could inhibit the growth of H. pylori. However there were no significant morphological changes of H. pylori. Many mycoproteins were regulated by Hydrotalcite. Without Hydrotalcite, a large amount of H. pylori attached on the cell surface of GES-1 promoted the apoptosis of GES-1 cell. Compared with blank control group, the apoptosis rate of GES-1 cells increased after treated by H. pylori ((5.52±1.31)% vs (28.96±3.14)%), and the difference was statistically significant (t=-11.94, P<0.05). Hydrotalcite could prevent the attachment of H. pylori on cells, which reduced the apoptosis caused by H. pylori ((28.96±3.14)% vs (19.38±1.91)%), and the difference was statistically significant (t=4.52, P<0.05). Conclusion Hydrotalcite has inhibitory effects on H. pylori in vitro and play a role in regulation of many mycoproteins. Hydrotalcite can reduce the attachment of H. pylori on gastric epithelial cells, and protect cells from H. pylori injury. Key words: Helicobacter pylori; Apoptosis; Hydrotalcite; Antibacterial activity; Mechanisms
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