Abstract
Retention in supercritical fluid chromatography (SFC) is determined by solute solubility in the fluid and solute interaction with the stationary phase. The functional relationship between retention and pressure at constant temperature has been described by Van Wasen and Schneider. The trend in retention is shown to depend on the partial molar volume of the solute in the mobile and stationary phase coupled with the isothermal compressibility of the fluid mobile phase. The purpose of this work was to determine the relationship between solubility and retention. A thermodynamic model was developed which predicts the trend in retention as a function of pressure, given the solubility of the solute in the fluid mobile phase. From this model, solute retention behavior can be examined by theory and experiment in order to gain some insight into the complicated dependence of retention on the thermodynamic and physical properties of the solute and the fluid, providing a basis for consideration of more subtle effects unique to SFC. 15 refs., 2 figs.
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