Abstract
In rat liver after bilateral adrenalectomy de novo purine biosynthesis was studied 24, 48 and 72 hours in comparison to sham-operated control. At these time points the rate of de novo purine biosynthesis assayed by [14C]glycine incorporation to hepatic purines significantly increased by 1.61, 1.95 and 2.01 folds respectively associated with comparable free glycine concentration in plasma or liver tissue. Hepatic concentration of 5-phosphoribosyl 1-pyrophosphate(PRPP) increased by 2.91, 1.50 and 1.75 folds at these 3 time points. 24 hours after bilateral adrenalectomy ATP and GTP concentration decreased by 33 and 24% respectively which were counterbalanced by the increased concentration of AMP(245%) and GMP(38%) associated with 18% decrease of adenylate energy charge. The replacement of corticosterone acetate to adrenal-ectomized animals for 24 hours partially reversed these changes. The results suggest that the increased rate of de novo purine biosynthesis in adrenal-ectomized rat liver is mediated by the increased concentration of PRPP and that it is compensatory against increased catabolism of purine ribonucleotides as a result of increased AMP concentration.
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