Abstract
Pigeon liver fatty acid synthetase multienzyme complex has been quantitatively dissociated in low ionic strength glycine-tris buffer into two subunits of identical or nearly identical molecular weight.A total of 5. 5 ± 1.0 readily titratable -SH groups/molecule are lost during the formation of the subunits. The dissociated enzyme is reconstituted to an active enzyme in high ionic strength buffer in the presence of dithiothreitol. The extent of reassociation is a function of the concentration of subunits. Under optimum conditions a complete recovery of enzyme activity is obtained.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Biochemical and Biophysical Research Communications
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
