Mechanism and protective effect of Dexamethasone on renal injury induced by ischemia-reperfusion in rats

Abstract

Objective To explore mechanism and protective effect of dexamethasone on renal injury induced by ischemia-reperfusion in rats. Methods The right kidney of rats was excised and the left renal artery was clamped with non-invasive clamp for 60 minutes to replicate the renal ischemia-reperfusion model. The successful rats were divided into model group and dexamethasone group (10 mg/kg). At the same time, the sham operation group was established (right kidney was not resected, left renal artery was not clamped). There were 10 rats in each group. Rats were scored for renal tubular injury. The level of malondialdehyde (MDA), the activity of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), the level of tumor necrosis factor alpha (TNF-α), interleukin (IL)-1β, IL-6, and the expression of nuclear factor-κ-gene binding κ (NF-κB) signal pathway related protein was detected. The variance analysis and LSD-t test are used in the data analysis. Results Compared with the model group, the renal tubular injury score [(41.56±4.16) vs. (85.32±8.53)] was decreased (t=14.580, P<0.01), the contents of TNF-α [(4.56±0.48) vs. (14.23±1.42)], IL-1β [(72.47±7.25) vs. (243.56±24.53)], IL-6 [(98.24±9.82) vs. (232.38±20.68)] and MDA [(47.34±4.73) vs. (84.24±8.42)] was decreased (t=20.400, P<0.01), the activities of SOD [(4.36±0.44) vs. (2.74±0.27)], CAT [(10.24±1.36) vs. (8.69±0.87)] and GSH-Px [(18.62±1.87) vs. (14.53±1.43)] was increased (t=9.920, P<0.01), and the expressions of p-IκBα [(0.42±0.03) vs. (0.98±0.10)] and p-NF-κB p65 [(0.39±0.04) vs. (1.95±0.15)] was decreased in dexamethasone group (t=16.960, P<0.01). Conclusion Dexamethasone might inhibit renal ischemia-reperfusion injury in rats through antioxidant and anti-inflammatory effects. Key words: Dexamethasone; Ischemia-reperfusion; Renal injury; Oxidant; Inflammation