STAR protocols | VOL. 3

Measuring trogocytosis between ovarian tumor andnatural killer cells.

Publication Date Jun 17, 2022


Trogocytosis is an active transport mechanism by which one cell extracts a plasma membrane fragment with embedded molecules from an adjacent cell in a contact-dependent process leading to the acquisition of a new function. Our protocol, which has general applicability, consolidates and optimizes existing protocols while highlighting key experimental variables to demonstrate that natural killer (NK) cells acquire the tetraspanin CD9 by trogocytosis from ovarian tumor cells. For complete details on the use and execution of this protocol, please refer to Gonzalez etal. (2021).


Active Transport Mechanism Ovarian Tumor Cells Natural Killer Ovarian Tumor Adjacent Cell Ovarian Killer General Applicability Killer Cells Ovarian Cells Natural Killer Cells

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No potential conflict of interest was reported by the authors. The conception and design of the study, acquisition of data, analysis and interpretatio...

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