Measuring the effectiveness of antibiotics against Pseudomonas aeruginosa and Escherichia coli that isolated from urinary tract infection patients in Al-Najaf city in Iraq

Abstract

Bacteria are the causative agent of human infection because of their ability to resistant to different antimicrobials due to it has different antimicrobial associated-genes. Therefore, this study aimed to determine the bacteria resistance to antibiotics. Bacteria isolated from patients with urinary tract infection in Al-Najaf City – Iraq. A total of 45 samples which were diagnosed by traditional morphological, biochemical tests, and Vitek2. During the period from October to December 2020. The Vitek2 system was the best method for diagnosis, which has led to isolating and diagnosis of 16/45 (35.55%) Pseudomonas aeruginosa and 4/45 (8.88%) Escherichia coli. Antimicrobial’s susceptibility testing was performed by disc diffusion, 11 antibiotics were used in this study. Polymerase chain reaction technique was used to detect blactx-m antimicrobial resistance-associated gene. The isolates of Pseudomonas aeruginosa have high sensitivity (100%) to Gentamicin, Ciprofloxacin (87.5 %), and Tetracycline (81.25%). While the isolates of Escherichia coli have high sensitivity (100%) to Ciprofloxacin and Tetracycline, (75 %) to Amikacin and Gentamicin. Out of the 16 isolates of Pseudomonas aeruginosa, there were 13 (81.25%) isolates that were positive for bla-ctxm gene. While the isolates of Escherichia coli, there were 4 (100%) isolates that were positive for bla-ctxm gene.