Abstract
A method for determining the concentration of available haptoglobin in plasma and other solutions is described. The procedure involves absorbance measurements in a mixture of plasma and hemoglobin before and after the addition of sodium dithionite. The haptoglobin content of the solution is calculated from the ratio of the absorbances at the Soret peaks of the oxy- and deoxyhemoglobin. The assay can be performed in minutes, and the error of measurement is well under 10%.
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