Abstract
A high-performance liquid chromatographic (HPLC) method for the determination of l-carnitine in clamped and frozen rat livers is described. L-carnitine + acetyl-CoA ⇆ acetyk-camitine + CoASH Using the above enzymatic reaction, release of CoASH is stoichiometric with the l-carnitine added. The present method has made possible the determination of carnitine in liver tissues, which is difficult by the conventional enzymatic spectrophotometric method using 5,5′-dithiobis(2-nitrobenzoic acid), owing to acetyl-CoA hydrolysis during prolonged incubations at pH 7.8.
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