Abstract
Fatty acid oxidation is usually measured by collecting CO from [C]-labelled lipid. An alternative technique is to estimate HO production from [H]-lipid substrate; this has been used in working rat heart with [H]fatty acid and [H]triacylglycerol. HO appearance was linear and rates of [H]oleate and [H]triolein oxidation similar to [C]palmitate and [C]tripalmitin oxidation. Measurement of [H]lipid oxidation by HO estimation is simple, accurate, and a practicable alternative to the CO technique.
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