Abstract
Certain patients, despite receiving proper treatment, still show higher failure rates of restorative dental treatments. The aim of this work was to test if MMP2 and MMP3 alleles are overrepresented in individuals with secondary caries. A total of 1,089 individuals from the University of Pittsburgh School of Dental Medicine Dental Registry and DNA Repository project were selected for this study. From this total, 341 individuals were selected for having a record of secondary caries in any type of restoration and were matched with 748 individuals by sex, age, ethnicity, and restorative work in the same teeth that did not fail. Genomic DNA extracted from saliva was used to obtain genotypes in five markers of MMP2 and MMP3 using TaqMan chemistry and end-point analysis. Chi-square was used to test if differences in allele and genotype distributions were statistically different at an alpha of 0.05. The less common allele and homozygote genotype of MMP2 rs9923304 were less commonly found among individuals with secondary caries. The less common allele of MMP2 rs2287074 was also less frequent among individuals with secondary caries. These results provide statistical evidence for the role of MMP2 in failure of restorations due to secondary caries. We can conclude that MMP2 variation impacts the risk of having secondary caries, independent of the restorative material.
Highlights
Dental caries, which is one of the most common diseases in both developing and industrialized countries, has a multifactorial etiology as well as being a chronic and complex disease [1]
We showed that variants in MMP2 and MMP3 were associated of having a deep caries lesion that involved a periapical lesion formation [9]
The less common allele and homozygotes genotype of MMP2 rs9923304 were less common among individuals with secondary caries
Summary
Dental caries, which is one of the most common diseases in both developing and industrialized countries, has a multifactorial etiology as well as being a chronic and complex disease [1]. Secondary caries is a major clinical problem defined as a carious lesion seen on the margins of the existing restoration or caries associated with sealants [3], and progress to the dentin and pulp. This form of caries has been reported in the literature as the most common reason for the replacement. Since the incidence of dental caries continues to be high among certain groups due to high sugar consumption and change of living conditions, there is a need to determine markers that will help identify individuals with a higher risk of caries [6]. The aim of this study was to test for over-representation of alleles in MMP2 and MMP3 markers to identify statistical evidence that these genes may influence longevity of dental restorative treatments
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