Abstract
SYNOPSIS. The use of axenic medium permits the study of mating type determination in stock 51 (sensitive) of syngen 4 of Paramecium aurelia. A high frequency of cytoplasmically bridged pairs was correlated with a high frequency of change of mating type following conjugation in axenic medium. The direction of change was predominantly from mating type VII to mating type VIII, suggesting a dominance of type VIII cytoplasm in the clones arising from a mixed cytoplasmic ancestry. No significant effect of either lower temperature or of NaX3 upon the pattern of mating type determination was found. The high frequency of cytoplasmic bridges between conjugants led to the formation of many double or higher multiplex clones.
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