Abstract
Faba bean is gaining attention in Australia as a rotation grain legume where most of the country’s produce is exported. Rust, caused by Uromyces viciae-fabae, is a major constraint to Faba bean production in eastern Australia and its chemical control results in increased cost of production. The deployment of diverse sources of resistance in new cultivars underpins economic and eco-friendly control of rust diseases of crops. A selection from cultivar Doza (Doza#12034) and a European accession Ac1655 exhibited seedling rust resistance against U. viciae-fabae pathotype 24–40. Doza#12034 and Ac1655 were crossed with a susceptible genotype Fiord and recombinant inbred line (RIL) F6 populations were generated. Rust tests on Fiord/Doza#12034 and Fiord/Ac1655 F4 and F6 populations demonstrated monogenic inheritance of resistance in both crosses and the underlying resistance loci were named Uvf-2 and Uvf-3, respectively. Genetic mapping of both RIL populations located Uvf-2 and Uvf-3 in chromosomes III and V, respectively. The SNPs that showed linkage with Uvf-2 and Uvf-3 were converted into kompititive allele specific PCR (KASP) assays. Markers KASP_Vf_0703 and KASP_C250539 flanked Uvf-2 at 4.9 cM and 2.9 cM distances, whereas Uvf-3 was flanked by KASP_Ac×F165 (2.5 cM) and KASP_vf_1090 (10.1 cM). Markers KASP_Vf_0703 and KASP_Ac×F165 can be used for marker-assisted selection of Uvf-2 and Uvf-3, respectively, after confirming parental polymorphisms.
Highlights
Faba bean (Vicia faba L.) is an early domesticated crop species and a source of protein for humans and animals
QTL for several desired economic traits of faba bean have been reported [30,44], markers linked with rust resistance did not get attention
Breeding rust resistant cultivars is the best disease management strategy where success relies on the identification of new resistance sources and their deployment into high yielding and well adapted agronomic backgrounds [20]
Summary
Faba bean (Vicia faba L.) is an early domesticated crop species and a source of protein for humans and animals. Ethiopia and Australia contribute more than 70% to the global faba bean production [4]. It was introduced in Australia in the late 20th century as a rotational crop after wheat and since been gradually established as an important legume crop because of its increasing export potential [5]. Australia is the world’s leading exporter of faba bean with an average of 218 thousand tons (69% of total produce) to Egypt and other Middle Eastern countries [6]. It plays a key role in soil fertility restoration through nitrogen fixation by its successful association with Rhizobium sp. It plays a key role in soil fertility restoration through nitrogen fixation by its successful association with Rhizobium sp. (root nodulation bacterium), making a significant contribution to agricultural sustainability [7]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
