Abstract
A method for mapping the 3′ ends of Ad-2 late nuclear RNA on the Ad-2 genome is described. Ad-2 DNA was digested with exonuclease III to expose sequences complementary only to the 3′ end portion of the late transcript. The positions of the late RNA hybridized to the ExoIII-treated Ad-2 DNA was determined by electron microscope heteroduplex analysis. The 3′ ends of the large late nuclear RNA were found to be within the right-most 2% of the Ad-2 genome. The results are consistent with previous analysis using the pulse-labeling technique that transcription of major late RNA of Ad-2 DNA continues 2 to 3 kilobases beyond the 3′ end of the most promoter distal mRNA before it is terminated. The occurrence of RNA termination sites distal to the poly(A) addition site is found to be a general feature of DNA tumor viruses SV40, polyoma, and adenoviruses.
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