Abstract
Early maturity is one of the most important agronomical traits in potato breeding. To identify the DNA segment that codes for early maturity, a tetraploid potato segregation population of “Zhongshu 19” × “Zhongshu 3” was genetically analyzed, using a combination of high throughput simplified genome sequencing (2b-RAD) and bulked segregant analysis (BSA). The DNA segment related to the early-maturity trait was identified at the 3.7~4.2 Mb locus on the short arm of chromosome 5. Eight molecular markers were developed, of which five were closely linked to the early-maturity trait loci. Additionally, 42 simple sequence repeats (SSR) markers were constructed based on the reference sequence of Solanum tuberosum group Phureja DM1-3 516 R44 (DM). Using the TetraploidMap software, the linkage map of chromosome 5 was constructed with 50 markers. The total map length was 172 centiMorgan (cM), with an average genetic distance of 3.44 cM. Correlating molecular and phenotypic data of the segregating population, the mapped Quantitative Trait Loci (QTL) on the short arm of chromosome 5 contributed to 33.55% of the early-maturity phenotype. The early-maturity QTL was located at 84 cM, flanked by the SSR5-85-1 and SCAR5-8 markers. The QTL was fine-mapped to 471 kb. Using DNA sequence annotation, 34 genes were identified in this region, 12 of them with unknown function. Among the other 22 annotated genes, E3 ubiquitin ligase gene PUB14 could be related to maturity and regulation of tuber formation. The constructed QTL map is a useful basic tool for the cloning of early-maturity related genes in tetraploid potatoes.
Highlights
Potato (Solanum tuberosum L.) is the third most consumed crop worldwide and is an important industrial raw material
Previous studies have shown that potato maturity was controlled by minor recessive polygenes, which were distributed in 12 chromosomes [1]; because diploid potatoes have simpler genetic ratios than tetraploids, it is easy for genetic analysis and molecular operations to be carried out
In Liaocheng (LC), the potatoes were planted in early March, emerged in mid-April, and some genotypes reached their physiological maturity in late June, while others were still growing and could not reach their physiological maturity due to very high temperatures
Summary
Potato (Solanum tuberosum L.) is the third most consumed crop worldwide and is an important industrial raw material. The development of potato markers and genetic map construction are mainly performed at diploid level, using traditional Quantitative Trait Loci (QTL) mapping, and most of the studies mapped the major QTL for maturity in chromosome 5, linked to the QTL for resistance to late blight [2,3,4,5,6]. In 2007, a diploid potato population was used to map a QTL associated with growth stages that is closely linked to marker BA47f2t7 (P1) in chromosome 5 [1]. A major maturity-associated QTL was identified in chromosome 5, which showed a contribution rate of 54.7% to the phenotype [9]. One QTL associated with maturity, closely linked to molecular marker c2_476095, was mapped in chromosome 5 with a contribution rate of 55% [11]. StCDF1.2 belongs to the DNA-binding One Zinc Finger transcription factor family and participates as an intermediate regulator factor in the potato tuber induction pathway
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