Abstract
Studies of fungal endophytes in tree leaves are largely descriptive and taxonomic. Testing hypotheses on the ecological role of these fungi is difficult as manipulating micro-organisms in the field or greenhouse is not easy. This study describes a technique which allows considerable manipulation of infection levels of endophytes within the leaves of the plant host without the use of fungicides. It involves placing bags (composed of clear PVC tops and polyester netting bottoms and sides) over branches so newly emerging leaves are protected from infective propagules. Leaves can be infected with target fungi by the repeated spraying of spore suspensions onto the leaves.
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