Manipulating the N release from 15N labelled celery residues by using straw and vinasses

Abstract

The aim of this laboratory study was to investigate the effect of straw and vinasses on the nitrogen (N) mineralization–immobilization turnover of celery residues during two periods (each simulating a time period from autumn till spring) under laboratory conditions. During the first period (1–198 d), 15N-labelled celery residues (1.1 g dry matter (DM) kg −1 soil) were incubated together with straw (8.1 g DM kg −1 soil), aiming to immobilize the N released from celery residues, followed by an incorporation of vinasses (1.9 g DM kg −1 soil) after 84 d, with a view to remineralizing the immobilized celery–N. During the second period (198–380 d), the experimental set-up was repeated, except that non-labelled celery residues were used. Total N, mineral N and their 15N enrichments as well as microbial biomass N were determined at regular time intervals. During both periods, mixing celery residues with straw significantly increased microbial biomass N (90.5 and 40.5 mg N kg −1 extra compared to celery only treatment) and decreased the amount of mineral N (reduction of 56.1 and 45.9 mg N kg −1 soil compared to celery only treatment) and the celery-derived mineral 15N (0% of mineral celery-derived 15N in straw treatment compared to 35% of mineral celery-derived 15N in celery only treatment). After maximum immobilization, a natural remineralization (without addition of vinasses) of 32.2 (at day 198) and 11.1 mg N kg −1 soil (at day 380) occurred in the straw treatment, but the mineral N content remained significantly lower than in the celery only treatment during the complete experiment, and the amount of remineralized celery– 15N was very low (5.4% of celery-derived 15N after 380 d). Vinasses caused no real priming effect, although it did slightly increase the amount of remineralized celery– 15N (+6.4% of celery-derived 15N at day 380 compared to the straw treatment), probably due an apparent added N interaction caused by displacement reactions with the soil microbial biomass.