Abstract
This study was undertaken to investigate the factor(s) released from cultured embryonic cells that is responsible for the prolonged mortility of bull spermatozoa and to determine some of the properties of the factor(s). Spermatozoa were incubated at 37°C in a supernatant removed from a 4-day culture medium of embryonic chick skeletal muscle cells. Their motility was maintained for 25.2 h. In contrast, spermatozoa incubated in the fresh medium rapidly lost their motility. A filtrate of the 4-day culture medium, obtained by passing the fluid through an Amicon YC-05 ultrafiltration membrane, retained a favorable effect on prolonging motility of spermatozoa. Heating or freezethawing of the filtrate did not interfere with the ability of the spermatozoa to maintain their motility. Oxygen consumption of spermatozoa in the filtrate of the 4-day culture medium was similar to that of spermatozoa in the fresh medium. These results suggest that a low molecular weight factor(s) (Mol. Wt. < 500) supplied by the cultured cells effectively prolonged the survival of the spermatozoa.
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