Abstract
The metallophyte Imperata cylindrica inhabits copper (Cu) polluted soils in large areas from Central Chile. Here, we subjected clonal vegetative plantlets to 300 mg Cu kg−1 of substrate for 21 days to identify the main molecular pathways involved in the response to Cu stress. Transcriptomic analyses were performed for shoots and roots, with and without Cu supply. RNA-Seq and de novo transcriptome assembly were performed to identify the gene response associated with molecular mechanisms of Cu tolerance in I. cylindrica. De novo transcriptome revealed a total of 200,521 transcripts (1777 bp) comprising ~91% complete ultra-conserved genes in the eukaryote and Plantae database. The differentially expressed genes (DEGs) in roots were 7386, with 3558 of them being up-regulated and the other 3828 down-regulated. The transcriptome response in shoots was significantly less, showing only 13 up-regulated and 23 down-regulated genes. Interestingly, DEGs mainly related with actin and cytoskeleton formation, and to a minor degree, some DEGs associated with metal transporters and superoxide dismutase activity in root tissues were found. These transcriptomic results suggest that cytoskeleton could be acting as a mechanism of Cu-binding in the root, resulting in a high Cu tolerance response in this metallophyte, which deserve to be analyzed ultra-structurally. Our study contributes to reinforcing the potential of I. cylindrica as a candidate plant species to be used as a phytoremediation agent in Cu-contaminated environments.
Highlights
Soil degradation originating from exacerbated mining activity has become a global environmental problem [1,2], which need to be solved in the short term
The Cu applied in the growth substrate of I. cylindrica plants generated a significant decrease in the fresh biomass production in both shoots and roots compared to the control conditions (Figure 1A,B) after 21 days of the treatment
Differential expression analysis using the de novo transcriptome of Imperata cylindrica plants growing under Cu stress generated the identification of a large number of genes involved in the Cu tolerance response, especially in root organs
Summary
Soil degradation originating from exacerbated mining activity has become a global environmental problem [1,2], which need to be solved in the short term. In Chile, Copper (Cu) mining has produced a strong deleterious impact, with large areas of soils contaminated with potentially toxic elements (PTEs), which highlights large amounts of Cu [3,4,5]. There are some plant populations capable to survive in soils with high levels of PTEs, known as metallophytes. These species can survive under high Cu soil levels; they can be implemented in phytoremediation processes [11,12]
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