Abstract

Recently, the applications in biotechnological and medical fields using nonequilbrium atmospheric pressure-plasmas (NEAPPs) have been intensively studied. The inactivation of microorganisms using NEAPPs has been attracted much attention due to the low temperature processing and high speed treatment. We inactivated Penicillium digitatum spores using an atmospheric pressure plasma in gas phase and focused on the effects of neutral oxygen radicals on the inactivation using an atmospheric-pressure oxygen radical source, which selectively supplies neutral oxygen radical species without charged species and UV radiation. We measured the densities of the neutral oxygen radicals, such as ground-state atomic oxygen (O(3Pj)) and singlet oxygen molecule (O2(1Δg)), using a vacuum ultraviolet spectroscopy system, and inactivated P. digitatum spores quantitatively. 3 The inactivation efficiencies corresponded to the O(3Pj) density. Therefore, we found quantitatively that O(3Pj) was the dominant factor responsible for inactivating P. digitatum spores.1

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