Abstract
Because zebrafish embryos are transparent, cell behaviors and interactions can be directly imaged noninvasively in live embryos using differential interference contrast-Nomarski light microscopy. We found that the imaging quality can be much improved by coupling differential interference contrast-Nomarski to true (analogical) color video so as to visualize the image in real time on a high-resolution colour video monitor. We explain here how to apply this approach to the in vivo imaging of embryonic macrophages, which constitute a distinct early macrophage lineage, that originates from the rostral-most lateral mesoderm--adjacent to the cardiac field, differentiate in the yolk sac, and rapidly spread in embryonic tissues, although still retaining proliferative capacity.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
