Abstract
MADS-box transcription factors are vital regulators participating in plant growth and development process and the functions of most of them are still unknown. ANR1 was reported to play a key role in controlling lateral root development through nitrate signal in Arabidopsis. OsMADS25 is one of five ANR1-like genes in Oryza Sativa and belongs to the ANR1 clade. Here we have investigated the role of OsMADS25 in the plant’s responses to external nitrate in Oryza Sativa. Our results showed that OsMADS25 protein was found in the nucleus as well as in the cytoplasm. Over-expression of OsMADS25 significantly promoted lateral and primary root growth as well as shoot growth in a nitrate-dependent manner in Arabidopsis. OsMADS25 overexpression in transgenic rice resulted in significantly increased primary root length, lateral root number, lateral root length and shoot fresh weight in the presence of nitrate. Down-regulation of OsMADS25 in transgenic rice exhibited significantly reduced shoot and root growth in the presence of nitrate. Furthermore, over-expression of OsMADS25 in transgenic rice promoted nitrate accumulation and significantly increased the expressions of nitrate transporter genes at high rates of nitrate supply while down-regulation of OsMADS25 produced the opposite effect. Taken together, our findings suggest that OsMADS25 is a positive regulator control lateral and primary root development in rice.
Highlights
Nitrogen (N) is one of the essential macronutrients required by plants for normal growth and development and is frequently the major limiting factor for crop yields [1]
Since OsMADS25 is closely related to ANR1 but is inducible by nitrate [38, 39], we investigated its possible role in NO3- regulation of root architecture, initially using Arabidopsis as a model system
Previous studies reported that the expression of OsMADS25 was significantly regulated by NH4+ [38], we further investigated whether OsMADS25 regulates primary and lateral root development in response to ammonium
Summary
Nitrogen (N) is one of the essential macronutrients required by plants for normal growth and development and is frequently the major limiting factor for crop yields [1]. In Arabidopsis, the ANR1 and AtABF3 genes are reported to be involved in distinct NO3- signaling pathways regulating LR development [26, 27]. Exogenous application of NO3- has been reported to affect the expression of ANR1, a MADS-box transcription factor in regulating LR numbers and LR elongation [26]. The root phenotype of the ANR1 overexpressing lines mainly depends on the presence of NO3-, suggesting that there other components involve in NO3--dependent signaling pathway. MiR444 has been reported to target four ANR1-like homologous genes (OsMADS23, OsMADS27a, OsMADS27b and OsMADS57) to regulate root development in rice [32,33,34,35,36]. To gain further insight into the possible regulatory functions of OsMADS25 in control root development in rice, we have investigated its regulatory role in root development through NO3- regulation
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