Abstract
Treatment in vitro of Ehrlich ascites tumor cells or human fibroblasts with 8-methoxypsoralen (8-MOP, 2.4 μM) and UVA irradiation results in a 30% and 60% respectively reduction in lysosomal β-galactosidase activity in situ. Under identical conditions one 8-MOP adduct was formed per 2 × 10 4 bases of DNA, one 8-MOP adduct was formed per ∼ 10 4 tRNA molecules and one per ∼ 100 ribosomes. It is suggested that the decrease in lysosomal β-galactosidase activity is a result of leakage through the lysosomal membrane caused by psoralen—UVA damage of the lipids in the membrane, since no effect was found on β-galactosidase in vitro. These results indicate that the lysosomes may also be a target for cellular photodamage by 8-methoxypsoralen.
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